His60 ni equilibration buffer
WebbHis60 Ni Superflowは、Hisタグの種類（6×His-、HN-、HAT-の各タグ）や変性、未変性の条件を問わずOne Stepでの精製を可能にする。 なお、目的Hisタグ融合タンパク質の …
His60 ni equilibration buffer
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WebbHis60 Ni Buffer Set). Table I: His60 Ni Superflow Resin Characteristics Capacity1 (mg protein/ml resin) up to 60 Matrix Superflow Bead size (µm) 60–160 Maximum linear flow … WebbHis60 Ni Superflow Cartridge는 His60 Ni Superflow Resin이 충진된 pre-packed FPLC cartridge로 AKTA나 FPLC 등의 LC system이나, syringe 또는 매뉴얼 방법을 이용한 his-tagged protein 정제에 사용할 수 있다. His60 Ni Superflow Cartridge Purification Kit 에는 His60 Ni Superflow Cartridges (1 ㎖× 5 ea 또는 5 ㎖× 5 ...
WebbClontech’s His60 Ni Superflow Cartridges are prefilled with 1 ml or 5 ml of His60 Ni Superflow resin and are ready to use for purification of his-tagged proteins using a syringe, peristaltic pump, or liquid chromatography systems such as ÄKTA or other FPLC systems. A. His60 Ni Superflow 1 ml & 5 ml Cartridge Specifications . Table 1. WebbHis60 Ni Superflow Resin是一个高容量的Ni-IDA树脂，可以从细菌、哺乳动物和杆状病毒感染的细胞中，一步纯化重组的His标签蛋白。 该产品属于改良型的Ni-IDA树脂，相比于其他Ni-NTA或Ni-IDA树脂来说，容量高、金属离子泄漏少，纯度高。 产品特点 ·高达60 mg/ml的His标签重组蛋白 ·洗脱的His标签重组蛋白中，金属离子泄露少 ·可在变性或非 …
Webb4 apr. 2024 · Equilibration Buffer: 50 mM sodium phosphate, 300 mM sodium chloride, 20 mM imidazole; pH 7.4 Wash Buffer: 50 mM sodium phosphate, 300 mM sodium … Webbinstructions on preparing Wash Buffer. NOTE: If you are using the buffers supplied in the HisTALON Buffer Set (Cat. No. 635651) or the HisTALON Gravity Column Purification …
WebbThe His60 Ni Buffer Set includes extraction, equilibration and elution buffers optimized for the purification of his-tagged proteins on His60 Ni Superflow Resin and His60 Ni …
WebbPanel A displays the elution profile of steps a to c . (B) A column containing 10 mL of pristine Ni 2+ pre-charged His60 Ni Superflow resin was rinsed with 10 CV of equilibration buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 20 mM imidazole, pH 7.4). ppt of digital marketingWebbHis60 Ni Elution Buffer: pH 7.4, ready to use, no dilution needed. Wash Buffer: Wash Buffer is easily made by mixing 710 μl of His60 Ni Elution Buffer with 9.29 ml of His60 … ppt of familyWebbClontech’s His60 Ni Superflow Resin is a high-capacity Ni-IDA resin that has been optimized for the efficient purification of expressed his-tagged proteins from bacterial, … pptoffice主题样式在哪设置WebbThe His60 Ni Gravity Column can be quickly regenerated by adding 20 ml of His60 Ni Equilibration Buffer or by washing with 10 column volumes of 20 mM MES, 0.3 M NaCl; pH 5.0 buffer. Regeneration allows the column to be reused to purify the same protein multiple times without significant loss of binding capacity. ppt office 2007Webb10 mM imidazole, pH 7.4) and 5 to 10 column volumes of distilled water before. recharging the column. Recharge the water-washed column by loading 0.5 ml or 2.5 ml of. 0.1 M NiSO4 in distilled ... pptoffice下载WebbHis60 Ni Superflow Cartridge Purification Kit (5 x 1 ml) provides 5 prepacked . His60 Ni Superflow Cartridges (each containing 1 ml of resin), as well as all the buffers needed … ppt office 2013http://m.manuals.plus/m/d0aa0fd973d1bd43ff3b40ae8fabec08f9a75592a8499730a808089df1aaafbf.pdf ppt office主题修改
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