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WebbTable 1 lists those chemical reagents not directly affecting the development of dye color. (Note: Basic buffer conditions and deter-gents interfere with this assay.) Since every … WebbThe biuret method is based on the fact that proteins (and, as a rule, all substances containing two or more peptidic bonds) react with copper to form a colored complex whose absorption ( λmax = 454 nm), in the presence of excess copper, is proportional to the amount of protein present. The reagent is obtained by dissolving 1–5 g of copper ... payoff document for property
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